Hieng-Ming Ting Assistant Professor
Ph.D. in Plant Sciences, Wageningen University, the Netherlands
Specialty: Plant molecular biology, Plant metabolomics
Laboratory: Life Science Building R932
Current Research Interests
1) Metabolic engineering and synthetic biology of plant metabolites (polyacetylenes, terpenes)
2) Study plant metabolites induced cellular responses and signal transduction in plants
3) Study role of volatiles organic compounds (VOCs) between different organism
Metabolic engineering and synthetic biology of plant metabolites (polyacetylenes, terpenes) Physiological roles of plant secondary metabolites could be reflected through their importance in plant defense against pathogens/predators and different environmental stresses, and as communication molecules when interact between different species. Many plants secondary metabolites are recognized to be major sources for (new) drug discovery, nutrition supplements and are often used as medicine against chronic diseases. Comparative transcriptomics and metabolomics technologies will be used to engineer and elucidate the biosynthesis pathway of plant secondary metabolites (polyacetylenes, terpenes) (Figure 1).
Figure 1. Workflow illustrating how to do metabolic engineering in plant systems.
Study plant metabolites induced cellular responses and signal transduction in plants
Plant secondary metabolites are synthesized upon the pathogen attack or mechanical damage, which cause the disruption of plant tissue. However, plant secondary metabolites are toxic for the plant at higher doses. To investigate the molecular mechanisms underlying the recognition/perception and signaling of plant secondary metabolites in plants, available Arabidopsis mutants will be treated with specific metabolites for further transcriptomics and metabolomics profiling studies (Figure 2).
Figure 2. (A) Phenotypes of Arabidopsis (Col) and mutant (Mut) under specific metabolite (GHP) treatment. (B) Transcriptional profiling analysis of Arabidopsis (Col) and mutant (Mut) under specific metabolite (GHP) treatment.
Bo Wang#, Arman Beyraghdar Kashkooli#, Adrienne Sallets, Hieng-Ming Ting, Norbert C.A. de Ruijter, Linda Olofsson, Peter Brodelius, Marc Boutry, Harro J. Bouwmeester, and Alexander van der Krol (2016) Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua. Metabolic Engineering, 38: 159–169. (# equal contribution)
Hieng-Ming Ting, Thierry L. Delatte, Pim Kolkman, Johana C. Misas-Villamil, Renier A.L. van der Hoorn, Harro J. Bouwmeester, and Alexander van der Krol (2015) SNARE-RNAi results in higher terpene emission from ectopically expressed caryophyllene synthase in Nicotiana benthamiana. Molecular Plant, 8(3):454–466.
Seifu Juneidi, Hieng-Ming Ting, and Alexander van der Krol (2014) Tissue specific expression of a terpene synthase in Nicotiana benthamiana leaves. American Journal of Plant Sciences, 5(18):2799-2810.
Hieng-Ming Ting, Bo Wang, Anna-Margareta Rydén, Lotte Woittiez, Teun van Herpen, Francel W.A. Verstappen, Carolien Ruyter-Spira, Jules Beekwilder, Harro J. Bouwmeester, and Alexander van der Krol (2013) The metabolite chemotype of Nicotiana benthamianatransiently expressing artemisinin biosynthetic pathway genes is a function of CYP71AV1 type and relative gene dosage. New Phytologist, 199(2):352–366.
Na Tian, Shuoqian Liu, Hieng-Ming Ting, Jianan Huang, Sander van der Krol, Harro Bouwmeester, and Zhonghua Liu (2013) An improved Agrobacterium tumefaciens mediated transformation of Artemisia annua L. by using stem internodes as explants. Czech Journal of Genetics and Plant Breeding, 49(3): 123–129.
Trends in Plant Science