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Hsu-Liang Hsieh Professor

  • Ph.D., University of Texas, at Austin, USA

  • Specialty: Plant Molecular Biology, Phytochrome Signal Transduction, Molecular Genetics

  • E-mail: hlhsieh@ntu.edu.tw

  • Laboratory: Life Science Building R919 Laboratory

  • TEL: 886-2-3366-2540

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Research Focus

  • Use the yeast two-hybrid system to screen Arabidopsis FIN219-interaction proteins.

  • Use transcriptional fusion to explore the regulatory mechanism of FIN219 gene expression

  • The role of FIN219 in far-red and blue-light message transmission

  • The method of activation tagging is used to screen the modifiers of FIN219 gene

Laboratory of Light Plant Physiology and Genetics

Plant development is regulated by both extrinsic factors, including light, and intrinsic factors such as genetics and phytohormones. We use Arabidopsis seedlings as a model system to study the integration of light- and multiple hormones-mediated signalings, leading to photomorphogenic development. FIN219/JAR1, a jasmonate conjugating enzyme that belongs to a GH3 gene family in Arabidopsis, has been shown to participate in phytochrome A (phyA)-mediated far-red (FR) light signaling and can be induced by auxin and jasmonate. FIN219 as an extragenic suppressor of COP1 can interact with different partners to regulate hypocotyl elongation under far-red light condition. Ectopic expression of different domains of FIN219 in wild-type Columbia shows that the N- and C-terminal domains have an opposite effect on photomorphogenic development. Furthermore, our data reveal that the fin219 mutant affects the levels of COP1 and HY5 specifically under FR, but not other light conditions. COP1 and HY5 interact in the dark, resulting in the degradation of HY5 and leading to a skotomorphogenesis with long hypocotyls and closed cotyledons. In contrast, in the light, COP1 exclusively moves to the cytoplasm and releases HY5 from repression, giving rise to a photomorphogenic development with short hypocotyls, open cotyledons and light responsive gene expression. How the FIN219/JAR1 expression affects the regulation of COP1 and HY5 interaction relationship remains to be elucidated. How jasmonate signaling mediated by FIN219/JAR1 modulates the photomorphogenesis involving COP1 and HY5 regulatory relation is also intriguing. Thus, we plan to use molecular genetics, cell biology and microarray approaches to dissect the functional role of FIN219/JAR1 in the crosstalk between FR light and jasmonate signaling pathways.

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FIN219 binds to COP1 protein and its possible function in Arabidopsis. The above picture uses the two-molecule fluorescent protein complementation method (upper left picture) and co-immunoprecipitation method (upper right picture) to prove that FIN219 protein can bind to COP1 protein; the picture below shows FIN219 and COP1 protein when Arabidopsis seedlings respond to light signals Combination and regulation of hypocotyl elongation model diagram.

Functional research on the effects of infrared radiation on plant growth and development

In addition to discussing the function of FIN219 / JAR1 in light and jasmonic acid messages, we also studied how invisible light, such as infrared light, affects the growth and development of Arabidopsis seedlings in the wavelength range of 3-5 μm. The results of previous laboratory studies have shown that Arabidopsis can sense infrared light and activate some signal transmission through the photosynthesis mechanism to inhibit the elongation of hypocotyls and increase biological weight. Recently, we have tried to understand the influence of infrared rays on plant photosynthesis, hoping to obtain more useful information and application value, which can reduce global warming and provide a better life for human beings.

To study the molecular mechanism of light-regulated lycopene content in tomato fruits. In addition to regulating the growth and development of plant seedlings, light messages also affect fruit growth, including the accumulation of lycopene in tomato fruits. We want to understand the molecular regulation mechanism of light-regulated lycopene content in tomato fruits of heat-resistant strains. For this purpose, the laboratory has established tomato transgenic plants that affect the content of lycopene in fruits, and has begun to explore the molecular regulation mechanism. It is hoped that in the near future, lycopene with high economic value and helpful to human health can be obtained. Tomato strain with rich vegetarian content.

To study the molecular mechanism of light-regulated lycopene content in tomato fruits

In addition to regulating the growth and development of plant seedlings, light information also affects fruit growth, including the accumulation of lycopene in tomato fruits. We want to understand the molecular regulation mechanism of light-regulated lycopene content in tomato fruits of heat-resistant strains. For this purpose, the laboratory has established tomato transgenic plants that affect the lycopene content of fruits, and has begun to explore the molecular regulation mechanism. It is hoped that in the near future, lycopene with high economic value and helpful to human health can be obtained. Tomato strain with rich vegetarian content.

Selected Publications

  1. Jiang HW, Peng KC, Hsu TY, Chiou YC, Hsieh HL (2023) Arabidopsis FIN219/JAR1 interacts with phytochrome a under far-red light and jasmonates in regulating hypocotyl elongation via a functional demand manner. PLOS Genetics 19(5): e1010779. https://doi.org/10.1371/journal.pgen.1010779

  2. Kai-Chun Peng, Wei Siao and Hsu-Liang Hsieh* (2023) FAR-RED INSENSITIVE 219 and phytochrome B corepress shade avoidance via modulating nuclear speckle formation. Plant Physiology. 00:1-17. https://doi.org/10.1093/plphys/kiad103

  3. Peng KC, Siao W, Hsieh HL* (2022) FAR-RED INSENSITIVE 219/JAR1 and phytochrome B co-repress shade avoidance in Arabidopsis via modulating nuclear speckle formation. Plant Physiol (Accepted)

  4. Wang CC, Hsieh HY, Hsieh HL, Tu SL.* (2021) The Physcomitrella patens. chromatin adaptor PpMRG1 interacts with H3K36me3 and regulates light-responsive alternative splicing. Plant Physiol. 185:1229-1241. doi: 10.1093/plphys/kiaa103.

  5. Shiah YJ*, Hsieh HL, Chen HJ, Radin DI. (2021) Effects of intentionally treated. water and seeds on the growth of Arabidopsis thaliana. Explore (NY) 17:55-59. doi: 10.1016/j.explore.2020.04.006.

  6. Jang GJ, Yang JY, Hsieh HL, Wu SH* (2019) Processing bodies control the selective translation for optimal development of Arabidopsis young seedlings. Proc Natl Acad Sci USA: https://doi.org/10.1073/pnas.1900084116

  7. He SL, Hsieh HL, Jauh GY* (2018) SMALL AUXIN UP RNA62/75 are required for the translation of transcripts essential for pollen tube growth. Plant Physiology, 178: 626-640; DOI: https://doi.org/10.1104/pp.18.00257.

  8. Huai-Ju Chen, Tsu-Yu Fu, Shao-Li Yang, Hsu-Liang Hsieh* (2018) FIN219/JAR1 and cryptochrome1 antagonize each other to modulate photomorphogenesis under blue light in Arabidopsis. PLOS Genetics March 21, 2018

  9. Shiah YJ*, Hsieh HL, Chen HJ, Radin DI. (2017) Effects of Intentionally Treated. Water on Growth of Arabidopsis thaliana Seeds with Cryptochrome Mutations. Explore (NY)13(6):371-378. doi: 10.1016/j.explore.2017.05.001.

  10. Swain S, Jiang HW and Hsieh HL* (2017) FAR-RED INSENSITIVE 219/JAR1 contributes to shade avoidance responses of Arabidopsis seedlings by modulating key shade signaling components. Front. Plant Sci. 8:1901.

  11. Chen CY, Ho SS, Kuo TY, Hsieh HL, and Cheng YS* (2017) Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation. PNAS 114 (10): E1815-E1824.

  12. Leu KC, Hsieh MH, Wang HJ, Hsieh HL, Jauh GY* (2016) Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing. RNA Biology 13: 593-604.

  13. Chen HJ, Chen CL, and Hsieh HL* (2015) Far-Red light-mediated seedling development in Arabidopsis involves FAR-RED INSENSITIVE 219/ JASMONATE RESISTANT 1-dependent and -independent pathways.PLoS ONE 10 (7): e0132723. doi:10.1371/journal.pone.0132723

  14. Lin LL, Hsu CL, Hu CW, Ko SY, Hsieh HL, Huang* HC and Juan HF* (2015) Integrating phosphoproteomics and bioinformatics to study brassinosteroid-regulated phosphorylation dynamics in Arabidopsis. BMC Genomics 16:533-549. DOI 10.1186/s12864-015-1753-4

  15. Hsu YW, Wang HJ, Hsieh MH, Hsieh HL and Jauh GY* (2014) Arabidopsis mTERF15 is required for mitochondrial nad2 intron 3 splicing and functional complex I activity. PLOS one 9: e112360. doi:10.1371/journal.pone.0112360.

  16. Hsieh HL and Okamoto* (2014) Molecular interaction of jasmonate and phytochrome A signaling. Journal of Experimental Botany 65: 2847-2857. doi: 10.1093/jxb/eru230

  17. Lin LL, Wu CC, Huang HC*, Chen HJ, Hsieh HL* and Juan HF* (2013) Identification of microRNA 395a in 24-epibrassinolide-regulated root growth of Arabidopsis thaliana using microRNA arrays. Int. J. Mol. Sci. 14: 14270-14286; doi:10.3390/ijms140714270.

  18. Liu XC, Chen CY, Wang KC, Luo M, Tai R, Yuan LY, Zhao ML, Yang SG, Tian G, Cui YH, Hsieh HL and Wu K* (2013) PIF3 associates with the histone deacetylase HDA15 in repression of chlorophyll biosynthesis and photosynthesis in etiolated Arabidopsis seedlings. Plant Cell 25: 1258-1273.

  19. Hsieh WP, Hsieh HL and Wu SH* (2012) Arabidopsis bZIP16 transcription factor integrates light and hormone signaling pathways to regulate early seedling development. Plant Cell 24: 3997-4011.

  20. Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL and Lin TP* (2012) Drought and salt stress tolerance of Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid. Plant Physiology 158: 340-351.

  21. Wang JG and Hsieh HL* (2012) Induction of tomato Jasmonate-Resistant 1-Like 1 gene expression can delay the colonization of Ralstonia solanacearum in transgenic tomato. Botanical Studies 53: 75-84.

  22. Wang JG, Chen CH, Chien CT and Hsieh HL* (2011) FAR-RED INSENSITIVE 219?? modulates CONSTITUTIVE PHOTOMORPHOGENIC 1 activity via physical interaction to regulate hypocotyl elongation in Arabidopsis. Plant Physiology 156: 631-646.

  23. Wu TH, Liao MH, Kuo WY, Huang CH, Hsieh HL and Jinn TL* (2011) Characterization of copper/zinc and manganese superoxide dismutase in green bamboo (Bambusa oldhamii): Cloning, expression and regulation. Plant Physiol Biochem 49: 195-200. ?(1: equal contribution)

Courses Taught

  • Photobiology

  • Plant Growth and Development

  • Plant Physiology

  • Special Topics in Plant Molecular Biology

  • Study on Plant Functional Genomics

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