吳克強 教授

E-mail: kewu@ntu.edu.tw
電話: (02) 3366-4546
專長: 表觀遺傳學, 植物生長與發育, 植物分子逆境反應
學歷: 加拿大薩斯喀徹溫大學博士

研究室: 生命科學館 1026 室

近年研究主題

植物生長發育及植物逆境反應的表觀遺傳 (epigenetic)機制
植物基因表達及調控 

研究室簡介

  • 表觀遺傳學  (epigenetics)是指由於非基因序列改變所引起的可遺傳的基因表達的變化。表觀遺傳的分子機理包括DNA的甲基化,組蛋白的修飾以及染色質的重塑 (chromatin remodeling)等。最近的研究表明, RNA 特別是非編碼 (noncoding) RNA 在表觀遺傳現象中也扮演重要的角色。植物生長與發育過程中的基因表達與染色質的結構的變化密切相關,而作為染色質的重要組成部分組蛋白上的蛋白質修飾在基因表達的調控上起重要的作用。組蛋白質修飾包括磷酸化﹐甲基化﹐ 乙醯化 (acetylation) 以及 ubiquitination 等。一般而言﹐若染色質的組蛋白乙醯化的程度較高, 則該部位的結構較為鬆散, 有利於基因的表達。反之, 若染色質的組蛋白乙醯化的程度較低, 則該部位的結構較為緻密, 不利於基因的表達。我們的研究發現, 組蛋白乙醯化與脫乙醯化參與了植物激素的信號傳導以及植物對逆境的反應。我們正在進一步研究組蛋白修飾對植物基因表達的影響以及在植物生長與發育的作用。這些研究將揭示植物生長與發育以及植物逆境反應過程中訊息傳導的分子機理。  

  • 阿拉伯芥是一種廣泛應用于分子生物學研究的模式植物。 阿拉伯芥的開花可以受到光周期調節。

圖中顯示野生型 (A, B) 以及突變體 (C, D) 阿拉伯 芥生長在長日照 (A, C) 或者短日照 (B, D) 條件下 的不 同開花時間。

代表著作

  1. Hung, F.‐Y., Chen, J.‐H., Feng, Y.‐R., Lai, Y.‐C., Yang, S. and Wu, K.*, Arabidopsis JMJ29 is involved in trichome development by regulating the core trichome initiation gene GLABRA3 . Plant J. May 2020.

  2. Yang, J., Yuan, L., Yen, M.‐R., Zheng, F., Ji, R., Peng, T., Gu, D., Yang, S., Cui, Y., Chen, P.‐Y., Wu, K.* and Liu, X., SWI3B and HDA6 interact and are required for transposon silencing in Arabidopsis . Plant J, 102: 809-822. 2020.

  3. Linmao Zhao, Tao Peng, Chia-Yang Chen, Rujun Ji, Dachuan Gu, Tingting Li, Dongdong Zhang, Yi-Tsung Tu, Keqiang Wu*, Xuncheng Liu. HY5 Interacts with the Histone Deacetylase HDA15 to Repress Hypocotyl Cell Elongation in Photomorphogenesis. Plant Phtsiology 180 (1) 
    May 2019

  4. Fu-Yu Hung, Fang-Fang Chen, Chenlong Li, Chen Chen, Jian-Hao Chen, Yuhai Cui* and Keqiang Wu* The LDL1/2-HDA6 Histone Modification Complex Interacts With TOC1 and Regulates the Core Circadian Clock Components in Arabidopsis. Front. Plant Sci., 26 February 2019

  5. Hung, F.-Y., F.-F. Chen, C. Li, C. Chen, Y.-C. Lai, J.-H. Chen, Y. Cui and K. Wu. (2018). The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes. NAR 46: 10669-10681.

  6. F.-Y. Hung, F.-F. Chen, C. Li, C. Chen, Y.-C. Lai, J.-H. Chen, Y. Cui, K.Wu*.​​The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes.Nucleic Acids Res. (2018) August; gky749-gky49. doi:10.1093/nar/gky749.

  7. Yu CW, Tai R, Wang SC, Yang P, Luo M, Yang S, Cheng K, Wang WC, Cheng YS and Wu K * (2017) HISTONE DEACETYLASE6 Acts in Concert with Histone Methyltransferases SUVH4, SUVH5, and SUVH6 to Regulate Transposon Silencing. Plant Cell. pii: tpc.00570.2016. doi: 10.1105/tpc.16.00570. [Epub ahead of print]

  8. Gu D, Chen CY, Zhao M, Zhao L, Duan X, Duan J*, Wu K * and Liu X* (2017) Identification of HDA15-PIF1 as a key repression module directing the transcriptional network of seed germination in the dark. Nucleic Acids Res. doi: 10.1093/nar/gkx283. [Epub ahead of print]

  9. Liu X, Luo M, Yang S and Wu K * (2015) Role of Epigenetic Modifications in Plant Responses to Environmental Stresses. In “Nuclear Functions in Plant Transcription and Signaling”, O. Pontes and H. Jin eds., Springer Science+Business Media, New York, pp., 81-92.

  10. Yang S, Li C, Zhao L, Gao S, Lu J, Zhao M, Chen C, Liu X, Luo M, Cui Yu, Yang C and Wu K *  (2015) Arabidopsis  SWI2 / SNF2 chromatin remodeling ATPase BRAHMA targets directly to pins and is required for root stem cell niche maintenance. Plant Cell 27:1670-1680.

  11. Luo M, Tai R, Yu CW, Yang S, Chen C, Lin WD, Wolfgan S and Wu K * (2015) Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis. Plant J 82:925-936.

  12. Zhao M, Yang S, Chen C, Li C, Shan W, Lu W, Cui Yu, Liu X, and Wu K * (2015) Arabidopsis BREVIPEDICELLUS interacts with the SWI2/SNF2 chromatin remodeling ATPase BRAHMA to regulate KNAT2 and KNAT6 expression in control of inflorescence architecture. PLoS Genet. 11: e1005125.

  13. Zhao M, Yang S, Liu X, and Wu K * (2015) Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating the DELAY OF GERMINATION 1 and ABA signaling-related genes. Front. Plant Sci. 6:159. doi: 10.3389/fpls.2015.00159.

  14. Li C, Chen C, Gao L, Yang S, Nguyen V, Shi X, Siminovitch K, Kohalmi SE, Huang S, Wu K, Chen X, Cui Y (2015) The Arabidopsis SWI2/SNF2 chromatin remodeler BRAHMA regulates polycomb function during vegetative development and directly activates the flowering repressor gene SVP. PLoS Genet.11: e1004944.

  15. Zhao L, Lu J, Zhang J, Wu P, Yang S, Wu K *(2015)Identification and characterization of histone deacetylases in tomato (Solanum Lycopersicum). Front. Plant Sci. 5:760. doi: 10.3389/fpls.2014.00760)

  16. Hsu C, Wu P, Chen T, Yu C, Tsai W, Wu K, Wu W, Chen W, and Chen H (2014) Histone acetylation accompanied with promoter sequences displaying differential expression profiles of B-class MADS-box genes for Phalaenopsis floral morphogenesis. PLoS ONE (accepted)

  17. Singh P, Yekondi S, Chen PW, Tsai CH, Yu CW, Wu K, Zimmerli L* (2014) Environmental history modulates Arabidopsis pattern-triggered immunity in a HISTONE ACETYLTRANSFERASE1-dependent manner. Plant Cell 26:2676-2688.

  18. Liu X, Yang S, Zhao M, Luo M, Yu CW, Chen C, Tai R, and Wu K* (2014) Transcriptional repression by histone deacetylases in plants. Mol Plant 7, 764–772.

  19. Luo M, Hung F, Yang S, Liu X, and Wu K* (2014) Histone lysine demethylases and their functions in plants. Plant Mol Biol Rep 32:558–565.

  20. Yuan L, Liu X, Luo M, Yang S, and Wu K* (2013) Involvement of histone modifications in plant abiotic stress responses. J Integr Plant Biol 55: 892-901.

  21. Liu X, Chen C, Wang K, Luo M, Tai R, Yuan L, Zhao M, Yang S, Tian G, Cui Y, Hsieh H and Wu K* (2013) PHYTOCHROME INTERACTING FACTOR3associates with the Histone Deacetylase HDA15 in repression of chlorophyll biosynthesis and photosynthesis in etiolated Arabidopsis seedlings. Plant Cell 25: 1258–1273.

  22. Zhou Y, Tan B, Luo M, Lin Y, Liu C, Chen C, Yu C-Wu, Yang S, Dong S, Ruan J, Yuan L, Zhang Z, Zhao L, Li C, Chen H, Cui Y,Wu K*and Huang S* (2013) HISTONE DEACETYLASE19 interacts with HSL1 and participates in the repression of seed maturation genes in Arabidopsis seedlings. Plant Cell 25: 134–148.

  23. Luo M, Yu C-W, Chen F-F, Zhao L, Tian G, Liu X, Cui Y, Yang J-Y, and Wu K* (2012) Histone Deacetylase HDA6 Is Functionally Associated with AS1 in Repression of KNOX Genes in Arabidopsis. PLoS Genet 8(12): e1003114. doi:10.1371/journal.pgen.1003114

  24. Luo M, Wang YY, Liu X, Yang S, Lu Q, Cui Y, and Wu K* (2012) HD2C interacts with HDA6 and is involved in ABA and salt stress response in Arabidopsis. J. Exp. Bot. 63: 3297-3306.

  25. Alinsug MV, Chen FF, Luo M, Tai R, Jiang L, and Wu K* (2012) Subcellular Localization of Class II HDAs in Arabidopsis thaliana: Nucleocytoplasmic Shuttling of HDA15 Is Driven by Light. PLoS ONE 7(2): e30846. doi:10.1371/journal.pone.0030846

  26. Liu X, Yu CW, Dun J, Luo M, Wang K, Tian G, Cui Y, and Wu K* (2012) HDA6 directly interacts with DNA methyltransferase MET1 and maintains transposable elements silencing in Arabidopsis. Plant Physiol. 158: 119-129

  27. Liu X, Luo M, Zhang W, Zhao J, Zhang J, Wu K*, Tian L* and Duan J* (2012) Histone acetyltransferases in rice (Oryza sativa L.): phylogenetic analysis, subcellular localization and expression. BMC Plant Biology 12:145. doi:10.1186/1471-2229-12-145

  28. Gao Y, Yang S, Yuan L, Cui Y, and Wu K* (2012) Comparative analysis of SWIRM domain-containing proteins in plants. Comp. Funct. Genomics 2012:310402.

  29. Luo M, Wang Y, Liu X, Yang S, Wu K*, (2012) HD2 proteins interact with RPD3-type histone deacetylases. Plant Signal Behav. 7: 608 – 610.

  30. Liu X, Luo M, and Wu K* (2012) Epigenetic interplay of histone modifications and DNA methylation mediated by HDA6. Plant Signal Behav. 7: 633 – 635

  31. Luo M, Liu X, Singh P, Cui Y, Zimmerli L, and Wu K* (2011) Chromatin modifications and remodeling in plant abiotic stress responses. Biochim. Biophys. Acta. – Gen. Regul. 1819: 129-136.

  32. Zhou Y, Chu P, Chen H, Li Y,Liu J,Ding Y,Tsang EW, Jiang L, Wu K, and Huang S. (2012) Overexpression of Nelumbo nucifera metallothioneins 2a and 3 enhances seed germination vigor in Arabidopsis.Planta (2012) 235:523–537

  33. Yu CW, Liu X, Luo M, Chen C, Lin X, Tian G, Lu Q, Cui Y, and Wu K* (2011) Histone Deacetylase6 interacts with Flower Locus D and regulates flowering in Arabidopsis. Plant Physiol. 156: 173-184.

  34. Chen L, and Wu K* (2010) Role of histone deacetylases HDA6 and HDA19 in ABA and abiotic stress response. Plant Signal Behav. 5: 1318-1320.

  35. Chen L, Luo M, Wang Y, and Wu K* (2010) Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response. J. Exp. Bot. 61: 3345-3353.

  36. Song Y, Wu K, Dhaubhade S, An L and Tian L (2010) Arabidopsis DNA methyltransferase AtDNMT2 associates with histone deacetylase AtHD2s activity. Biochem. Biophys. Res. Commun. 396:187-192.

  37. Fu G, Zhong Y, Li C, Li Y , Lin X, Liao B, Tsang E WT, Wu K* , and Huang S (2010) Epigenetic regulation of peanut allergen gene Ara h 3 in developing embryos. Planta 231: 1049-1060.

  38. Li C, Wu K, Fu G, Li Y, Zhong Y, Lin X, Zhou Y, and Huang S (2009) Regulation of oleosin expression in developing peanut embryos through nucleosome loss and histone modifications. J. Exp. Bot. 60: 4371-4382.

  39. Alinsug MV, Yu C-W and Wu K* (2009) Phylogenetic analysis, subcellular localization, and expression patterns of RPD3/HDA1 family histone deacetylases in plants. BMC Plant Biol. 9: 37.

  40. Wu K* , Zhang L, Zhou C, Yu C, and Chaikam V (2008) HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis. J. Exp. Bot.59: 225–234.

  41. Liu Z, Zhou C and Wu K (2008) Creation and analysis of a novel chimeric promoter for the complete containment of pollen- and seed-mediated gene flow. Plant Cell Rep. 27:995–1004

  42. Ciou C, Wu K. and Yeh K. (2008) Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium gower ramsey. Biotechnology Letters 30:1861-1866.

  43. Fu W., Wu K. and Duan J. (2007) Sequence and expression analysis of histone deacetylases in rice. Biochem. Biophys. Res. Commun. 356:843-850.

開設課程

  • 生物技術核心實驗

  • 植物生理學

  • 植物分子生物學研究方法

  • 植物科學專題研究討論

國立臺灣大學植物科學研究所 National Taiwan University Institute of Plant Biology

+886-2-3366-2525~6    ntuplant@ntu.edu.tw

地址: 10617 臺北市大安區羅斯福路四段 1 號   臺大生命科學館 307 室

address: Room 307, Life Science Building, NTU. No. 1, Sec. 4, Roosevelt Rd., Taipei, Taiwan 10617