E-mail: jinnt@ntu.edu.tw
電話: (02) 33662539
專長: 植物生理與生化、植物生長與發育、植物分子生物
學歷: 國立臺灣大學博士
研究室: 生命科學館913室
近年研究主題
植物熱休克蛋白質及熱逆境
植物超氧岐化酶及抗氧化機制
功能性基因體調控植物生長及發育
植物生理及功能性基因研究室
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熱逆境反應是普遍存在於生物界中,從原核生物到酵母菌、果蠅、植物、人類等都存在,是一種具高度保留性的生命現象。許多證據都支持熱逆境下所新產生的熱逆境蛋白質,可以用來保護細胞免受高溫或其他逆境之傷害。植物因無法自由遷移,所以當面臨高溫逆境時,勢必演化出較動物複雜的機制以便能適應此逆境,而植物如何抵抗逆境的機制,一直是學者們想要了解的課題。本研究室藉由分子生物學的角度,著重在研究熱休克蛋白質基因成員、生理功能、表現量、及對調控子及調控單元的研究,深入探討植物如何適應及克服環境高溫的不利因素,並利用遺傳工程技術希,望培育出更具耐性的農作物品種。
超氧自由基,是所有生物在正常有氧代謝過程中自然產生的活性氧分子,會造成氧化逆境,產生連鎖反應,破壞細胞,是老化及疾病的元兇。超氧岐化酶為超氧自由基的清除者,可解除氧化逆境。超氧歧化酶依其鑲嵌金屬的不同,可分成銅鋅、鐵及錳超氧岐化酶。這些金屬輔酶為超氧歧化酶活性所必須,但皆為過渡金屬,若為游離態時會造成細胞的傷害。針對這些金屬離子兩極化的需求,預測細胞中應有專一性的金屬鑲嵌蛋白的存在,並認為超氧歧化酶需經由這些鑲嵌蛋白的作用,將這些離子傳遞至超氧歧化酶而使其活化。我們利用分子遺傳學方法,分離、鑑定並探討這些金屬鑲嵌蛋白分子之生化特性。期望研究的成果能提供更多活化超氧歧化酶之重要線索,也提供細胞中金屬離子衡定之相關訊息及想法,希望能增強植物的抗氧化能力及延緩老化現象。
代表著作
A. 學術期刊論文:
- Huang Y.C., Liu C.C., Li Y.J., Liao C.M., Vivek S., Chuo G.L., Tseng C.Y., Wu Z.Q., Shimada T., Suetsugu N., Wada M., Lee C.M.* and Jinn T.L.* (2024) Multifaceted roles of Arabidopsis heat shock factor binding protein in plant growth, development, and heat shock response. Environmental and Experimental Botany 226, 105878.
- Silamparasan D., Chang I.F., and Jinn T.L.* (2023). Calcium-dependent protein kinase CDPK16 phosphorylates serine-856 of glutamate receptor-like GLR3.6 protein leading to salt-responsive root growth in Arabidopsis. Frontiers in Plant Science 14: 1093472.
- Hu S.-H. Hu and Jinn T.-L.*(2022) Impacts of Mn, Fe, and Oxidative Stressors on MnSOD Activation by AtMTM1 and AtMTM2 in Arabidopsis. Plants, 11(5), 619
- Wu, H. C., Yu, S. Y., Wang, Y. D., & Jinn, T. L. (2022). Guard Cell-Specific Pectin METHYLESTERASE53 Is Required for Abscisic Acid-Mediated Stomatal Function and Heat Response in Arabidopsis. Frontiers in Plant Science, 346.
- Hu, S. H., Lin, S. F., Huang, Y. C., Huang, C. H., Kuo, W. Y., & Jinn, T. L.* (2021). Significance of AtMTM1 and AtMTM2 for mitochondrial MnSOD activation in Arabidopsis. Frontiers in Plant Science, 12.
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Wu H.C., Vignols F., Jinn T.L.* (2019) Temperature Stress and Redox Homeostasis: The Synergistic Network of Redox and Chaperone System in Response to Stress in Plants. In: Asea A., Kaur P. (eds) Heat Shock Proteins in Signaling Pathways. Heat Shock Proteins, vol 17. pp 53-90. Springer, Cham.
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Victor P. Bulgakov*, Hui-Chen Wu, Tsung-Luo Jinn. (2019) Coordination of ABA and Chaperone Signaling in Plant Stress Responses.Trends in Plant Science 24: 636-651.
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Wu H.C., Bulgakov V.P. and Jinn T.L.* (2018) Pectin methylesterases: cell wall remodeling proteins are required for plant response to heat stress. Frontiers in Plant Science 9: 1612. doi: 10.3389/ fpls.2018.01612
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Wu H.C., Huang Y.C., Stracovsky L. and Jinn T.L.* ( 2017 ) Pectin methylesterase is required for guard cell function in response to heat. Plant Signaling and Behavior 12: e1338227. DOI: 10.1080/ 15592324.2017.1338227.
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Wu H.C., Huang Y.C., Liu C.H. and Jinn T.L.* ( 2017 ) Using silicon polymer impression technique and scanning electron microscopy to measure stomatal aperture, morphology, and density. Bio-protocol 7: e2449. DOI: 10.21769/BioProtoc.2449.
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Huang Y.C., Wu H.C., Wang Y.D., Liu C.H., Lin C.C., Luo D.L. and Jinn T.L.* ( 2017 )Pectin Methylesterase34 contributes to heat tolerance through its role in promoting stomatal movement. Plant Physiology 74: 748-763.
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Huang Y.C., Niu C.Y., Yang C.R. and Jinn T.L.* ( 2016 ) The heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses. Plant Physiology 172: 1182-1199.
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Kuo W., Huang C., Shih C. and Jinn T.L.* ( 2013 ) Cellular extract preparation for superoxide dismutase ( SOD ) activity assay. Bio-protocol 3: e811.http://www.bioprotocol.org/e811
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Xia S., Cheng Y.T., Huang S., Win J., Soards A., Jinn T.L., Jones J.D., Kamoun S., Chen S., Zhang Y. and Li X. (2013) Regulation of transcription of nucleotide-binding leucine-rich repeat-encoding genes SNC1 and RPP4 via H3K4 trimethylation. Plant Physiology 162: 1694-1705.
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Kuo W.Y., Huang C.H., Liu A.C., Cheng C.P., Li S.H., Chang W.C., Weiss C., Azem A. and Jinn T.L.* (2013) CHAPERONIN 20 mediates iron superoxide dismutase (FeSOD) activity independent of its co-chaperonin role in Arabidopsis chloroplasts. New Phytologist 197: 99-110.
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Kuo W.Y., Huang C.H. and Jinn T.L.* (2013) Chaperonin 20 might be an iron chaperone for superoxide dismutase in activating iron superoxide dismutase (FeSOD).Plant Signaling and Behavior 8: 2, e23074.
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Wu H.C., Luo D.L., Vignols F. and Jinn T.L.* (2012) Heat shock-induced biphasic Ca2+ signature and OsCaM1-1 nuclear localization mediate downstream signalling in acquisition of thermotolerance in rice (Oryza sativa L.). Plant Cell and Environment 35: 1543-1557.
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Wu H.C. and Jinn T.L.* (2012) Oscillation regulation of Ca2+/calmodulin and heat-stress related genes in response to heat stress in rice (Oryza sativa L.). Plant Signaling and Behavior 7: 1056-1057.
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Huang C.H., Kuo W.Y., Weiss C, and Jinn T.L.* (2012) Copper chaperone-dependent and -independent activation of three copper-zinc superoxide dismutase homologs localized in different cellular compartments in Arabidopsis. Plant Physiology 158: 737-746.
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Huang C.H., Kuo W.Y. and Jinn T.L.* (2012) Models for the mechanism for activating copper-zinc superoxide dismutase in the absence of the CCS Cu chaperone in Arabidopsis. Plant Signaling and Behavior 7: 429-431.
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Wu T.H., Liao M.H., Kuo W.Y., Huang C.H., Hsieh H.L. and Jinn T.L.* (2011)Characterization of copper/zinc and manganese superoxide dismutase in green bamboo (Bambusa oldhamii): Cloning, expression and regulation. Plant Physiology and Biochemistry 49: 195-200.
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Wu H.C., Hsu S.F., Luo D.L., Chen S.J., Huang W.D., Lur H.S. and Jinn T.L.* (2010)Recovery of heat shock-triggered released apoplastic Ca2+ accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings. Journal of Experimental Botany 61: 2843-2852.
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Wu H.C. and Jinn T.L.* (2010) Heat shock-triggered Ca2+ mobilization accompanied by pectin methylesterase activity and cytosolic Ca2+ oscillation are crucial for plant thermotolerance. Plant Signaling and Behavior 5: 1252-1256.
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Hsu S.F., Lai H.C. and Jinn T.L.* (2010) Cytosolic-localized heat shock factor binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis.Plant Physiology 153: 773-784.
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Hsu S.F. and Jinn T.L.* (2010) AtHSBP functions in seed development and the motif is required for subcellular localization and interaction with AtHSFs. Plant Signaling and Behavior 5: 1042-1044.
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Cho S.K., Larue C., Chevalier D., Wang H., Jinn T.L., Zhang S. and Walker J.C. (2008) Regulation of floral organ abscission in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 105: 15629-15634 (+Equal Contribution).
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Chang P.F.L., Jinnt T.L., Huang W.K., Chang H.M. and Wang C.W. (2007) A cDNA clone from rice (Oryza sativa L.) encoding a class II small heat shock protein is induced by heat stress, mechanical injury, and salicylic acid. Plant Science 172: 64-75.
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Chu C.C., Lee W.C., Guo W.Y., Pan S.M., Chen L.J., Li H-m. and Jinn T.L.* (2005) A copper chaperone for superoxide dismutase that confers three types of CuZnSOD activity in Arabidopsis thaliana. Plant Physiology 139: 425-436.
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Guan J.C., Jinn T.L., Yeh C.H., Feng S.P., Chen Y.M. and Lin C.Y. (2004)Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.). Plant Molecular Biology 56: 795-809.
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Jinn T.L., Chou C.C., Song W.W., Chen Y. M. and Lin C.Y. (2004) Azetidine induced accumulation of class I low-molecular-weight heat shock proteins in the soluble fraction provide thermotolerance in soybean seedlings. Plant and Cell Physiology45: 1759-1767.
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Jinn T.L., Stone J.M., and Walker J.C. (2000) HAESA, an Arabidopsis leucine-rich repeat receptor kinase, controls floral organ abscission. Genes and Development 14: 108-117.
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Jinn T.L., Chang P., Chen Y.M., Key J.L. and Lin C.Y. (1997) Tissue-type-specific heat-shock response and immunolocalization of class I low-molecular-weight heat-shock proteins in soybean. Plant Physiology 114: 429-438.
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Jinn T.L., Chen Y.M. and Lin C.Y. (1995) Characterization and physiological function of class I low-molecular-mass, heat-shock protein complex in soybean. Plant Physiology 108: 693-701.
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Lee Y.L., Chang P.F., Yeh K.W., Jinn T.L., Kung C.C., Lin W.C., Chen Y.M. and Lin C.Y. (1995) Cloning and characterization of a cDNA encoding an 18.0-kDa class-I low-molecular-weight heat-shock protein from rice. Gene 165: 223-227.
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Yeh K.W., Jinn T.L., Yeh C.H., Chen Y.M. and Lin C.Y. (1994) Plant low-molecular-mass heat-shock proteins: their relationships to the acquisition of thermotolerance in plants. Biotechnology and Applied Biochemistry 19: 41-49.
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Jinn T.L., Wu S.H., Yeh C.H., Hsieh M.H., Yeh Y.C., Chen Y.M. and Lin C.Y. (1993) Immunological kinship of class I low-molecular-mass heat shock proteins and thermostabilization of soluble proteins in vitro among plants. Plant Cell Physiology34: 1055-1062.
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Lin C.Y., Jinn T.L., Hsieh M.H., Yeh Y.C. and Chen Y.M. (1993) Class I low molecular weight heat shock proteins in plants: immunological study and thermoprotection against heat denaturation of soluble proteins. Biochemical and Cellular Mechanism86: 140-155.
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Hsieh M.H., Chen J.T., Jinn T.L., Chen Y.M. and Lin C.Y. (1992) A class of soybean low molecular weight heat shock proteins: immunological study and quantitation. Plant Physiology 99: 1279-1284.
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Jinn T.L., Yeh Y.C., Chen Y.M. and Lin C.Y. (1989) Stabilization of soluble proteins in vitro by heat shock proteins-enriched ammonium sulfate fraction from soybean seedlings. Plant Cell Physiology 30: 463-469.
B. 國內外學術研討會論文或摘要:
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Wang Y.H. and Jinn T.L. (2019) RNA binding protein GRP7 and 8 are capable for cytoplasmic-destined FeSOD activation in Arabidopsis. Plant Physiology, Poster Number: 1100-033.
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Huang Y.C. and Jinn T.L. (2017) The heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses. Plant Physiology, Poster Number: 183323.
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Jinn T.L. (2015) Pectin methylesterase34, PME34, contributing to stomata movement, is required for heat stress response in Arabidopsis. Plant Physiology, Poster Number: 1000-049-Y.
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Liu Chin-Cheng, Jinn T.L. (2014) Interaction profile of heat shock factor binding protein, a negative regulator of heat shock response, under heat shock and recovery stages. Plant Physiology, Poster Number: P04031-B.
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Huang C.H., Kuo W.Y., Jinn T.L. (2013) CPN20, an CPN60 cofactor, acts as an iron chaperon for SOD independently of chaperonin-mediated protein folding activity in Arabidopsis chloroplasts. Plant Physiology, Abstract P01032.
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Huang C.H., Kuo W.Y., Jinn T.L. (2012) Models for the mechanism in CCS-independent activation pathway. Plant Physiology, Abstract P09033.
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Hsu S.F., Lai H.C., and Jinn T.L. (2011) Cytosolic-localized heat shock factor binding protein, AtHSBP, functions as a negative regulator of heat shock response by translocation to the nucleus and is required for seed development in Arabidopsis.Plant Physiology. Abstract P07069
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Kuo W.Y., Huang C.H., and Jinn T.L. (2010) Arabidopsis cpn20 acts as an iron chaperone for FeSOD activation in chloroplasts independently of its co-chaperonin function. Plant Physiology. Abstract P08095
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Huang C.H., Kuo W.Y., and Jinn T.L. (2009) Arabidopsis copper-zinc superoxide dismutase (SOD) can be activated by copper chaperone for SOD1-independent pathway via glutathione. Plant Physiology. Abstract P27016
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Cho S.K., Chevalier D., Lease K., Jinn T.L., and Walker J. (2007) HAESA and HAESA-like 2 activate floral organ abscission in an ethylene-independent manner. Plant Physiology. Abstract P28048
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Wu H.C. and Jinn T.L. (2007) A versatile calmodulin is temperature-dependent sensor. Plant Physiology. Abstract P01030
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Guan J.C., You J.W., Yeh C.H., Jinn T.L., and Lin C.Y. (2006) A comprehensive analysis of rice small heat shock protein gene family. Plant Physiology. Abstract P09027
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Wu H.C. and Jinn T.L. (2006) The mobilizes of Ca2+ from extracellular sources that induced by heat shock to regulate cell wall remodeling and signaling to confer thermotolerance in rice seedlings. Plant Physiology. Abstract P09037
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Hsu S.F. and Jinn T.L. (2005) Study of a serrate leaf mutant caused by cyclin-dependent kinase inhibitors overexpressed in Arabidopsis. Plant Physiology. Abstract 692
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Chang P.F.L., Huang W.G., Jinn T.L., Wang C.W., Lin P.L., and Chang H.M. (2005) The class II small heat shock gene of rice (Oryza sativa L.), Oshsp18.0-CII, is induced by heat stress, mechanical injury, and also confers tolerance to heat and UV stresses when overexpressing it in Escherichia coli. Plant Physiology. Abstract 178
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Chu C.C. and Jinn T.L. (2004) Copper chaperone for superoxide dismutase modulates the activity and stability of CuZnSOD in Arabidopsis thaliana. Plant Physiology. Abstract 094
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Chang C.C. and Jinn T.L. (2004) Study of T-DNA tagged mutants which affect inosine-uridine nucleoside hydolase gene expression in Arabidopsis. Plant Physiology. Abstract 324
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Guan J.C., Feng S.P., Jinn T.L., and Lin C.Y. (2004) The Expression profile of rice class I sHSP gene family in response to cytotoxic agents induced HS-like response. Plant Physiology. Abstract 127
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Li Y.H. and Jinn T.L. (2003) A flavin monooxygenase-like overexpression mutant with narrow, down-curing and long petioles phenotypes in Arabidopsis. Plant Physiol. Abstract 455
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Guan J.C., Feng S.P., Jinn T.L., and Lin C.Y. (2003) Expression profile of nine members of rice class I sHSP gene family. Plant Physiology. Abstract 201
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Guan J.C., Feng S.P., Jinn T.L., and Lin C.Y. (2003) Identification and expression analysis of class I small heat-shock protein gene family in rice (Oryza sativa cv. Tainung No.67) seedlings. Cell Biology. Abstract B508
C. 學術專書:
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Wu H.C., Vignols F. and Jinn T.L.* Temperature Stress and Redox Homeostasis: The Synergistic Network of Redox and Chaperone System in Response to Stress in Plants.. Heat Shock Proteins in Signaling Pathways. Heat Shock Proteins. (ISBN: 978-3-030-03952-3). Springer, Cham.. Jul, 2019: vol 17.
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基因工程與生物技術-基因選殖及DNA分析。 (Gene cloning and DNA analysis. 5th edition, T. A. Brown) 編譯者: 靳宗洛;何國傑;葉開溫;鄭石通。 藝軒圖書出版社 (2008, 400 pages)
開設課程
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普通植物學
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分子生物學
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植物生長與發育
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逆境植物學
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植物分子生物學專論
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植物基因釣取